hMSCs Proliferation assay. In vitro expansion of AM-hMSC. Passage three AM-hMSCs were seeded at an initial concentration of 1000 cells/cm2 (time 0). At day 4, cells were harvested, counted with a hemocytometer and then re-plated at sub-confluent density. The same procedure was repeated at days 7, 10, 14, 17 e 21 (A). Comparison between growth kinetics of amniotic membrane derived cells and bone marrow derived cells. The figure shows the mean numbers of hMSCs obtained by hemocytometer counting on days 0, 4, 7, 10, 14, 17, 21. At t = 0 1000 cells per cm2 were seeded in 6 well plates. Triplicate cultures were harvested for each point. The values represent the mean and SD of three separate experiments (B).