Oct-4 expression. RT-PCR analysis of Oct-4 expression in 5th passage AM-hMSCs and BM-hMSCs. Samples are as follows: M: Marker; P: Positive control (HeLa cells); BM: BM-hMSCs; AM: AM-hMSCs; N: Negative control (reagent control). Beta-actin was used as a house-keeping gene. Amplicon lengths: Beta actin 236 bp; Oct-4 249 bp. The data are representative of a set of at least three experiments.