Figure 8

Nrg3 signaling stimulates sebocyte differentiation. (A and B) In non-transgenic epidermis (A), Oil Red O stains the sebaceous gland (indicated by arrows) associated with each hair follicle. In K14-Nrg3 skin (B), the sebaceous glands (indicated by arrows) are enlarged. (C) Section stained with H&E from non-transgenic littermate at P52. (D) Section stained with H&E from K14-Nrg3 transgenic skin at P52. Note the excessive sebaceous differentiation. (E and F) In non-transgenic epidermis (E), c-Myc expression is restricted to a few basal cells (indicated by arrows). In K14-Nrg3 skin (F), it is expressed throughout most of the basal epidermis (indicated by arrows). (G) Western blot for c-Myc. Lysates were prepared from ventral skin of non-transgenic (lane 1) and K14-Nrg3 littermates (lane 2) and blotted with antibodies against c-Myc and β-Tubulin (loading control). The scale bar represents 50 μm.