Figure 3

Dynamic expression of Dnmt3L in male germ cells. A) Relative quantification of Dnmt3L expression in postnatal male germ cells. Real-time RT-PCR was used to determine the expression profile of Dnmt3L in primitive type A (PA), type A (A) and type B (B) spermatogonia, preleptotene (PL), leptotene/zygotene (L/Z), prepubertal pachytene (PP) and pachytene (P) spermatocytes, as well as round spermatids (RS) and residual bodies/elongating spermatids (RB). Expression was determined in triplicate in each of the two series of germ cells and normalized to 18S expression; normalized results were calibrated to expression in pachytene spermatocytes. Shown here are the mean expression results obtained for one series. Mean ± SD. B) Relative quantification of Dnmt3L expression in prenatal male germ cells. Quantitative RT-PCR was used to measure the expression levels of Dnmt3L in total RNA extracted from E13.5, E15.5 and E18.5 prospermatogonia and 6 dpp primitive type A spermatogonia (PA). Expression of Dnmt3L was determined in triplicate in each of the two series of germ cells and normalized to 18S expression; normalized values were calibrated to the expression found in E13.5 gonocytes. Shown here are the mean expression results obtained for one series. Mean ± SD.