Figure 6

St. 29/30 (E6.5) Sympathetic neurons require NT3 and NGF for survival. St. 29/30 (E6.5) sympathetic ganglia were dissociated and sympathetic neurons were cultured as described in Methods. The cells were treated without neurotrophins (closed square), or with NT3 alone (open circle), NGF alone (closed triangle), or NT3 and NGF (closed diamond). The number of neurons in the same well was counted each day to assess survival. The neurons survive when cultured with both NT3 and NGF. Data represent mean ± SEM from three independent experiments. *Denotes statistical significance (p < 0.05), Two-way ANOVA.