Figure 4
From: A role for Phospholipase D in Drosophilaembryonic cellularization
Drosophila Pld recombinant protein localizes to cytoplasmic vesicles and the plasma membrane in COS7 cells. COS7 cells were transiently transfected with either pCGN:dPld, pCGN:mPLD1, or pCGN:mPLD2. The cells were immunostained with a rat monoclonal anti-HA antibody (3F10). mPLD1 localized to peri-nuclear vesicles, while mPLD2 was predominantly localized to the plasma membrane. Drosophila Pld staining marked intracellular vesicles and the plasma membrane.