Figure 7

Expression of Amnos in honeybee ovaries. Scale bars represent 100 micrometers. A) Ovariole from a mated queen bee stained for Amnos RNA. Amnos RNA is present in both oocytes and a subset of nurse cells. In oocytes in the late vitellarium, Amnos RNA is present in a localised domain in the posterior regions of the oocyte, with a 'tail' of Amnos RNA spreading back along one side of the oocyte towards the anterior. In the early vitellarium, Amnos RNA is localised in a domain in central regions of each oocyte. Amnos is also expressed weakly in the nurse cells, particularly in those closest to the oocyte. B) DIC image of an oocyte and nurse cells from the early vitellarium showing the localised domain of Amnos and the nucleus (asterisk). The patch of Amnos RNA is not associated with the nucleus. C) DIC image of the basal germarium of an ovariole stained for Amnos RNA. In the germarium, oocytes contain Amnos RNA. In late germarium, this is in a patch as seen in the vitellarium. In earlier oocytes, expression appears uniform. The patch of Amnos RNA is not associated with the oocyte nucleus (asterisks). No expression of Amnos is seen in the nurse cells. D) DIC image of an oocyte and nurse cells from the late vitellarium. Amnos RNA is only present in a small posterior patch, not associated with the nucleus (asterisk). The nurse cells all express Amnos weakly. E and F) DIC images of two focal planes through an oocyte in the vitellarium stained for Amnos RNA. Amnos RNA is present in a streak running down the oocyte, with the highest and most defined expression in the posterior. The streak and domain is not associated with the oocyte nucleus (asterisk in E). G) Worker bee ovariole stained for Amnos RNA. Strong expression is seen in the nurse cells of the vitellarium, with weaker expression in the oocyte. In the germarium, Amnos RNA is weakly expressed in oocytes only. No expression is present in the terminal filament (data not shown).