Figure 2

Analysis of Apis nanos. A) i) Map of the genomic region that contains the highest similarity to nanos sequences in the bee genome. The map indicates the regions of similarity to nanos genes, the cDNA region cloned and the position of putative bearded boxes associated with nanos translation regulation in Drosophila. ii and iii) RNA structures of the first 130 bases of the Drosophila nanos (ii) and Amnos (iii) 3'UTRs predicted by MFOLD [42]. B) Bayesian phylogeny of nanos protein sequences derived from the alignment shown in D. The predicted Apis mellifera nanos protein sequence (Amnos, underlined) clusters with Drosophila and Nasonia nanos sequences with high posterior probability. C) Alignment of the most conserved regions of nanos protein sequences from metazoa.