Figure 1

Analysis of Apis vasa. A) Bayesian phylogeny of vasa protein sequences and Rm62-like DEAD box helicases from insects (and Danio). The predicted Apis mellifera vasa protein sequence clusters with other insect vasa proteins to the exclusion of Rm62 DEAD box helicases. ENSANGP00000013029 and ENGSANG00000015773 are Anopheles predicted protein sequences. This phylogram was derived from a multiple alignment shown in C. B) Neighbour joining phylogeny of vasa protein sequences and honeybee predicted DEAD-box containing proteins. Amvasa is the only Apis DEAD-box containing gene to cluster within the vasa clade. C) Multiple alignment of the most conserved domains of Vasa predicted proteins. The region boxed in red is the EXRKF motif that is not conserved in Honeybee vasa. The region boxed in black is the predicted protein encoded by the 3' end of the probe used for in-situ hybridisation.