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Table 2 Effect of solubilised human zona pellucida on intracellular Ca2+ concentration and the acrosome reaction (AR) of capacitated spermatozoa

From: Zona pellucida from fertilised human oocytes induces a voltage-dependent calcium influx and the acrosome reaction in spermatozoa, but cannot be penetrated by sperm

   [Ca2+]i (nM) Ca2+ influx(nM) AR
Type of [Ca2+] increase Treatment Basal level Peak Sustained phase Peakb Sustained phasec (%) ZPIARd (%)
Pattern I (n = 12) hZPa 386.7 ± 35.2 1162.1 ± 196.5*** 1030.8 ± 232.4** 775.4 ± 190.8*** 644.2 ± 215.4** 61.4 ± 7.7* 44.0 ± 6.5
  Control 345.8 ± 42.5 363.7 ± 39.3 380.8 ± 33.2 17.9 ± 15.9 35.0 ± 15.5 17.4 ± 2.1  
Pattern II (n = 9) hZPa 487.4 ± 62.7   1188.9 ± 230.2**   701.4 ± 178.3** 60.9 ± 6.6* 34.9 ± 5.8
  Control 441.1 ± 42.9   467.8 ± 52.0   26.7 ± 25.0 26.0 ± 2.6  
  1. All the values are expressed as means ± SEM; the number of experiments is shown in brackets (n=); [Ca2+]i = intracellular Ca2+ concentration; AR = acrosome reaction; pattern I corresponds to a biphasic increase in [Ca2+]I; pattern II corresponds to a monophasic increase in [Ca2+]i ; a2 ZP/μl were incubated with the spermatozoa; bΔ between [Ca2+]i at peak and basal level; cΔ between [Ca2+]i at sustained phase and basal level; dhuman zona pellucida-induced acrosome reaction; *,**,*** statistically different from respective control *p < 0.05,**p < 0.01,***p < 0.001 with Student's t test.