Figure 3

Regions flanking the amos gene support GFP expression in all embryonic locations in which amos is expressed. (A–C) amos-3.5-GFP in early embryos, GFP expression (green) relative to Amos (magenta). (A) Stage 10. Most areas of Amos expression overlap with GFP, but arrows indicate two areas of Amos expression that are not mimicked by this reporter gene construct. (B) Stage 11. dbd (blue) and dmd1 (yellow) clusters are circled. (C) Stage 12. Arrow indicates a third region with no overlap of expression. (D) amos-D-GFP, stage 10. GFP is present in Amos-expressing clusters that correspond to the three areas arrowed in A and C. (E–G) amos-3.5-GFP in stage 15 embryos showing perdurance of GFP (green) relative to the sensory neuron marker, 22C10 (magenta). dmd1 and dbd neurons are arrowed in G. (H) amos-3.5-GFP in sca-Gal4 UAS-amos embryo. Widespread misexpression of Amos (magenta) does not result in ectopic expression of GFP (green) from this reporter construct. Abbreviations as in Fig. 1.