Figure 2

Enhancer elements flanking the amos gene. (A) Schematic of the amos genomic location summarising the locations of nearest neighbouring genes, fragments tested for enhancer activity, location of enhancer activity, and location of potential proneural protein-binding E box sites. (B–D) Antennal imaginal discs, 8 h after puparium formation. (B) amos-3.5-GFP, GFP (green) is almost completely coexpressed with Amos (magenta). stained to detect GFP (green), Amos (blue) and Sens (red) as an SOP marker. (C, D) GFP (green) is coexpressed in some cells with Amos (blue) and Sens (red) in amos-3.5-GFP (C) and amos-C-GFP (D). Expression of amos-C-GFP is lower than that of amos-3.5-GFP, although this is not apparent from these images.