RNAi epistasis analysis of age-1(mg109) suppressors reveals interdependence of AKT-1 and PDK-1. To establish whether akt-1(mg247), pdk-1(mg261) and mg227 mutations suppressed dauer arrest through the AGE-1/PI3 kinase pathway we carried out a series of genetic epistasis experiments using RNAi. Bars show the percentage of animals that bypassed dauer arrest, developing into L4 larvae or adults (grey), and those that arrested as dauers (black). Numbers on the side of the bars represent the total number of animals examined. RNAi experiments were diluted with control L4440 RNAi to match the mixed akt-1 and akt-2 RNAi dose. (A) age-1(mg109);akt-1(mg247) animals arrested as dauer larvae on pdk-1 RNAi. Similarly, age-1(mg109);pdk-1(mg261) animals arrested as dauer larvae on akt RNAi. (B) Epistasis analysis on age-1(mg109);mg227 animals suggests mg227 requires akt-1 but not akt-2, sgk-1 or pdk-1 to bypass dauer larval arrest.