Mutations identified as suppressors of constitutive dauer arrest in age-1(mg109) animals. Suppressors were mapped using standard SNP-snip-mapping protocols with the Hawaiian strain CB4856 . Mutations were confirmed using PCR and direct DNA sequencing. (A) daf-16(mg242) is a Trp220Amber substitution in exon 5, affecting both daf-16a and daf-16b splice forms. daf-16(mg255) is a Trp144Amber substitution in exon 3 that only affects the daf-16a splice form. Both mutations fall within the Forkhead domain (indicated by the grey area) involved in DNA interaction. (B) akt-1(mg247) is an Ala149Thr substitution that falls in the linker region, between the pleckstrin homology domain (PH, indicated by the grey boxes) and the protein kinase C domain (KD, indicated by the black boxes). (C) pdk-1(mg261) is a Ala447Val substitution in the linker region and a Glu530Trp substitution in the pleckstrin homology domain (PH, grey boxes) of PDK-1.