Figure 1

Concentrations of IGF-I and IGF-II in the conditioned media. The maternal and fetal intestinal fibroblasts produced primarily IGF-II. In this representative experiment the mean and SEM data represents the pooled data from triplicate samples from the three cell lines. The fetal fibroblasts produced greater concentrations of IGF-I and IGF-II than maternal fibroblasts but did not achieve statistical significance (8.4 ± 1.7 vs. 5.1 ± 0.03 ng/ml IGF-I, p = 0.53 and 40.3 ± 3.0 vs. 29.99 ± 1.3 ng/ml IGF-II, p = 0.08). The one week post-confluence fetal fibroblasts had increased IGF-I secretion (13.9 ± 0.4), while that of the maternal fibroblasts decreased (2.7 ± 0.3,* p < 0.001). The IGF-II secretion was decreased slightly one week post-confluence in fetal fibroblasts (29.9 ± 2.3) and significantly in maternal fibroblasts (to 9.5 ± 0.8, ^p < 0.05).