Nile Red staining of fat stores in wild-type, eat-3 , pha-2 and pha-3 adults. L4 worms were grown on NGM plates containing the lipid-specific dye Nile Red for 24 hrs. The arrows point at the most anterior part of the intestine. (A) In wild type worms the fat granules in the intestine fluoresced brightly. In (B) eat-3 (C) pha-2 and (D) pha-3 mutants the fluorescence was markedly decreased, indicating reduced levels of fat deposits.