Figure 2

Expression of activated DRok induces eye and wing defects. (A-C) Scanning Electron Microscopy photographs of wild-type (A), one-copy GMR-Drok-cat transgenic (B) or two-copy GMR-Drok-cat transgenic (C) eyes. Expression of one copy of DRok-cat results in a slightly rough eye (B), of two copies in a rougher eye phenotype (C), which provides a basis for a convenient assay to identify genetic modifiers. (D-F) Tangential retinal sections of wild-type (D), one-copy GMR-Drok-cat transgenic (E) or one-copy GMR-Drok-cat and one-copy GMR-p35 (F) eyes. In the one copy-transgenic eye, the underlying retina is severely disrupted, associated with loss of cells (E, arrow). This phenotype can be rescued by overexpressing the baculoviral caspase inhibitor p35 (F). (G, H) Light microscopy photographs of a wild-type (G) or a en-GAL4<UAS-Drok-cat expressing (H) wing. Expression of DRok-cat in the posterior part of the wing results in the disappearance of the crossveins (H, arrows).