Figure 2From: The contractile vacuole in Ca2+-regulation in Dictyostelium: its essential function for cAMP-induced Ca2+-influxEnrichment of V-type H+-ATPase in P 0 E. Western blot analysis with antibodies to V-type H+-ATPase was carried out as described in Methods using fractions of cells expressing dajumin-GFP. 7 μg of protein per lane of each fraction were applied.Back to article page