Figure 2
From: RCAS-RNAi: A loss-of-function method for the developing chick retina
In vivo test of RCAS-RNAi activity on visinin transcript expression. (A) A diagram of the RCAS-RNAi vectors tested. The hairpins were cloned in the forward and reverse directions in order to assess whether orientation altered virus knock down capabilities. (B) Infection of chick retinas with RCAS-U6-Vis1 and RCAS-U6-Vis2 in vivo. Stage 10 (~E1.5) retinas were injected with virus and harvested at E6. Retinas were hybridized with anti-visinin probe and then stained with 3C2 (anti-gag) antibody to allow visualization of virus-infected regions.