Figure 1

In vitro test of U6-Visinin vector activity on Visinin protein expression

(A) A diagram of the BS/U6 vector, showing the murine RNA Polymerase III-specific U6 promoter, the location for oligonucleotide cloning which code for the hairpin, and the 5 consecutive thymidine bases which serve as a termination sequence for RNA Polymerase III.

(B) Portions of the visinin sequence used to make hairpins. Four sequences depicted in red were chosen from the coding sequence. Each sequence started with three guanine bases, and each sequence was 21 nucleotides long. Oligonucleotides coding for these sequences were cloned into the BS/U6 vector as described in the Materials and Methods.

(C) Electroporation of chick retinas with BS/U6-Vis constructs in vitro. E6 retinas were electroporated with each construct and a GFP construct, and then were cultured for two days. Retinas were then dissociated and GFP positive cells were scored for Visinin expression.