Figure 5

The effect of variables on proliferation. Cell counts from 36–40 h.a.o. cell cultures, with varying number of initial eggs and with/without FBS. X-axis displays number of days after initiation of cultures, y-axis displays number of cells. Two cultures were initiated on the same number of embryos, whereupon one was added FBS (5, 10 or 15 %) whereas the other were not. Cultures initiated from 15 embryos, supplemented with FBS (15 %) were most successfully maintained. Generally, cultures which were reared in medium with FBS achieved higher proliferation rates.