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Figure 8 | BMC Developmental Biology

Figure 8

From: Aberrant stalk development and breakdown of tip dominance in Dictyosteliumcell lines with RNAi-silenced expression of calcineurin B

Figure 8

Analysis of chimeras formed from mixtures of AX2 and 01–5 cells. Cell lines were mixed in the ratios indicated and allowed to develop on HABP filters.

A. Gradual reduction of stalk size in chimeras containing increasing fractions of cells from line 01–5. A side view of the mature fruiting bodies on HABP filters at low magnification shows that stalk length gradually decreases with increasing fractions of 01–5 cells in chimeras.

B. Formation of ectopic tips in chimeras containing increasing fractions of cells from line 01–5. The fractions of fruiting bodies with ectopic tips on an entire HABP filter were counted in a blinded manner. Average values ± SEM from two independent experiments are shown.

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