Figure 7
Expression of ecmB mRNA in AX2 and 01–5 cells. Total RNA was prepared from cells developed on HABP filters for the times (in hours) indicated, separated on 0.9% agarose/6.6% formaldehyde gels, blotted onto nylon membranes and hybridized with a DIG-labelled probe for ecmB. A probe for actin15 was used as loading control (signals in the lower part). Note that expression of actin15 decreases during development.