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Table 2 Survival following microinjection or sham procedures.

From: High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivoin mice

age at intervention E6.5 E7.5 E7.5 E7.5 E7.5 E7.5
target EPC EPC Exo AC Sham 1 Sham 2
Embryo 2–12 d postinjection
# mice 10 8 6 15 6 nd
# concepti 119 76 64 145 90 nd
# alive 93 68 50 98 60 nd
% alive 78 89 † 78 68 67 nd
95% CI for % alive 70–85 80–95 66–87 59–75 56–76 nd
At birth
# mice 4 nd nd 3 5 5
# concepti 56 nd nd 43 61 62
# alive 41 nd nd 26 34 52
% alive 73 nd nd 60 56 * 84
95% CI for % alive 60–84 nd nd 40–75 42–68 72–92
1 wk Postnatal
# mice 4 2 nd 3 5 5
# concepti 56 29 nd 43 61 62
# alive 38 19 nd 26 33 52
% alive 68 66 nd 60 54 * 84
95% CI for % alive 54–80 46–82 nd 44–75 41–67 72–92
  1. nd = not done; # = number; CI = confidence interval; EPC = ectoplacental cone region; Exo = exocoelemic cavity; AC = amniotic cavity; Sham 1 = surgical procedure and ultrasound imaging without microinjection; Sham 2 = brief surgery to count embryos; † = % alive significantly greater than for AC and Sham 1; * = % alive in Sham 1 is significantly less than in Sham 2