High resolution ultrasound-guided microinjection for interventional studies of early embryonic and placental development in vivoin mice

Table 2 Survival following microinjection or sham procedures.

age at intervention	E6.5	E7.5	E7.5	E7.5	E7.5	E7.5
target	EPC	EPC	Exo	AC	Sham 1	Sham 2
Embryo 2–12 d postinjection
# mice	10	8	6	15	6	nd
# concepti	119	76	64	145	90	nd
# alive	93	68	50	98	60	nd
% alive	78	89 †	78	68	67	nd
95% CI for % alive	70–85	80–95	66–87	59–75	56–76	nd
At birth
# mice	4	nd	nd	3	5	5
# concepti	56	nd	nd	43	61	62
# alive	41	nd	nd	26	34	52
% alive	73	nd	nd	60	56 *	84
95% CI for % alive	60–84	nd	nd	40–75	42–68	72–92
1 wk Postnatal
# mice	4	2	nd	3	5	5
# concepti	56	29	nd	43	61	62
# alive	38	19	nd	26	33	52
% alive	68	66	nd	60	54 *	84
95% CI for % alive	54–80	46–82	nd	44–75	41–67	72–92

nd = not done; # = number; CI = confidence interval; EPC = ectoplacental cone region; Exo = exocoelemic cavity; AC = amniotic cavity; Sham 1 = surgical procedure and ultrasound imaging without microinjection; Sham 2 = brief surgery to count embryos; † = % alive significantly greater than for AC and Sham 1; * = % alive in Sham 1 is significantly less than in Sham 2

ISSN: 1471-213X