Figure 3

The levels of cleaved Dl intracellular domain is not associated with high levels of Da. A. Western blots (from a 8% SDS-PAGE) showing the level of Dl and DlIC in the indicated cell mixtures, with (+) or without (-) Sca. B. Western blots (from a 12% gel) showing the levels of Dl and DlIC in S2-Dl cells treated medium containing different levels of Sca. C. Western blots showing the levels of Da in the indicated cell mixtures. D. Western blots showing the levels of Da, Dl, and DlIC at different times following heat shock induction of Dl in S2-Dl cells. All experiments were repeated at least three times.