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Table 1 Information on the primers used for real-time PCR

From: Selection of reference genes for quantitative real-time PCR in bovine preimplantation embryos

Gene Genbank Accession number species Sequence Product size (bp) Ta (°C) % homology
ACTB [23] AY141970 Cow 5'-CCTCACGGAACGTGGTTACA-3' 5'-TCCTTGATGTCACGCACAATTT-3' 87 58 100 %
GAPD XM_618013 Cow 5'-TTCAACGGCACAGTCAAGG-3' 5'-ACATACTCAGCACCAGCATCAC-3' 119 62 100 %
Histone H2A [15] U62674 Mouse 5'-GTCGTGGCAAGCAAGGAG-3' 5'-GATCTCGGCCGTTAGGTACTC-3' 182 60 82 %
TBP [33] NM_003194 Human 5'-CCTAAAGACCATTGCACTTCG-3' 5'-CTTCACTCTTGGCTCCTGTG-3' 146 57 94 %
HPRT1 AF176419 Cow 5'-TGCTGAGGATTTGGAGAAGG-3' 5'-CAACAGGTCGGCAAAGAACT-3' 154 58 100 %
SDHA NM_174178 Cow 5'-GCAGAACCTGATGCTTTGTG-3' 5'-CGTAGGAGAGCGTGTGCTT-3' 185 60 100 %
YWHAZ BM446307 Cow 5'-GCATCCCACAGACTATTTCC-3' 5'-GCAAAGACAATGACAGACCA-3' 120 60 97 %
18S rRNA AF176811 Cow 5'-AGAAACGGCTACCACATCCA-3' 5'-CACCAGACTTGCCCTCCA-3' 169 62 100 %