Factors regulating the numbers of GH146-glia within the third segment of the antenna. (A,B) GH146::GFP /DER-lacZ stained with anti-β-galactosidase (red in A,B) and anti-GFP (green in B). Merged image in (B) shows that GH146-glia express the DER. (C-G). 36 hr APF antenna stained with anti-GFP (green) and anti-Repo (red). Cell numbers shown in each panel are from at least 10 antenna. (C) GH146::GFP/UAS-DN-DER. (D) UASRasN17/+; GH146::GFP/+ (E) GH146::GFP/UAS-DN-DER; UAS-P35/+ (F). GH146::GFP/UAS-Cdc42v12 (G) UAS-p21/+; GH146::GFP/+. (H,I) 36 hour APF antennae of GH146::GFP/UAS-DN-DER (H) and GH146::GFP/ UAS-Cdc42v12 stained with mAb22C10 (blue). ORNs exit the third segment of the antenna in three fascicles denoted I, II and III. The slightly distorted fasciculation pattern seen in (I) is within normal variation. The arista (Ar) is to the right in each figure. (J) Numbers of GH146-glia in antenna expressing different transgenes. Cells in 10 antennae were counted in each case and the mean and standard deviation is represented.