Figure 6

Representative TUNEL assays showing the effects of zygotic XR11 expression on radiation tolerance of ( A-F ) early post-MBT embryos and ( G-L ) late post-MBT embryos derived from eggs of wild-type females. A-F. Embryos were irradiated at stage 11.5 and fixed for TUNEL assay 12 hours later. A-C. GFP-negative embryos. A. Control. B. 10 Gy. C. 20 Gy. D-F. GFP-positive embryos (containing the XR11 transgene). D. Control. E, 10 Gy. F. 20 Gy. This experiment was conducted three times using sperm from five XR11 males. G-L. Embryos were irradiated in the mid-30 stages and fixed for TUNEL assay 12 hours later. G-I. GFP-negative embryos. G. Control. H. 10 Gy. I. 20 Gy. J-L. GFP-positive embryos (containing the XR11 transgene). J. Control. K. 10 Gy. L. 20 Gy. This experiment was conducted once using sperm from two XR11 males. The experiment could not be repeated due to the unavailability of additional XR11 males.