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Table 2 Sequences and annealing temperatures for the primer sets used in RT-PCR

From: Induction of chondro-, osteo- and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages

Gene Primer sequence Tm in °C
Chondrocyte-specific   
Aggrecan 5'-GATCTGGCATGAGAGAGGCG-3'
5'-GCCACGGTGCCCTTTTTAC-3'
61
Collagen type II 5'-GCTGCTGACGCTGCTCATC-3'
5'-GGTTCTCCTTTCTGCCCCTT-3'
60
Collagen type X 5'-CAAGCCAGGCTATGGAAGTC-3'
5'-AGCTGGGCCAATATCTCCTT-3'
60
Link protein 5'-TTCTGGGCTATGACCGCTG-3'
5'-AGCGCCTTCTTGGTCGAGA-3'
60
Biglycan 5'-CATGACAACCGTATCCGCAA-3'
5'-ATTCCCGCCCATCTCAATG-3'
60
Decorin 5'-ATGACCCTGACAATCCCCTG-3'
5'-CCCAGATCAGAACACTGCACC-3'
60
Scleraxis 5'-GGACCGCAAGCTCTCCAAG-3'
5'-ACCCACCAGCAGCACATTG-3'
62
Sox9 5'-GCAGACCAGTACCCGCATCT-3'
5'-CTCGCTCTCGTTCAGCAGC-3'
62
Adipocyte-specific   
ADD1 5'-CAGTGACTCTGAGCCCGACA-3'
5'-ATGCCTCGGCTATGTGAAGG-3'
61
PPARγ 5'-ATCATCTACACGATGCTGGCC-3'
5'-CTCCCTGGTCATGAATCCTTG-3'
59
SCD1 5'-ACACCATGGCGTTCCAAAAT-3'
5'-CGGCGTGTGTTTCTGAGAACT-3'
61
C/EBPα 5'-CGCAAGAGCCGAGATAAAGC-3'
5'-GCGGTCATTGTCACTGGTCA-3'
60
GLUT-4 5'-ATGGCTGTCGCTGGTTTCTC-3'
5'-ACCCATAGCATCCGCAACAT-3'
59
AP2 5'-TGATGCCTTTGTGGGAACCT-3'
5'-GCAAAGCCCACTCCCACTT-3'
58
acrp30 5'-AAGAAGGACAAGGCCGTTCTC-3'
5'-GAGGAGCACAGAGCCAGAGG-3'
60
LPL 5'-CCAATGGAGGCACTTTCCAG-3'
5'-CCACGTCTCCGAGTCCTCTC-3'
60
  1. Forward primer sequence is depicted from the 5' to the 3' end followed by the reverse primer. The appropriate annealing temperature for each set is given as Tm in °C.