Table 2 Sequences and annealing temperatures for the primer sets used in RT-PCR

Chondrocyte-specific

Aggrecan

5'-GATCTGGCATGAGAGAGGCG-3'

5'-GCCACGGTGCCCTTTTTAC-3'

61

Collagen type II

5'-GCTGCTGACGCTGCTCATC-3'

5'-GGTTCTCCTTTCTGCCCCTT-3'

60

Collagen type X

5'-CAAGCCAGGCTATGGAAGTC-3'

5'-AGCTGGGCCAATATCTCCTT-3'

60

Link protein

5'-TTCTGGGCTATGACCGCTG-3'

5'-AGCGCCTTCTTGGTCGAGA-3'

60

Biglycan

5'-CATGACAACCGTATCCGCAA-3'

5'-ATTCCCGCCCATCTCAATG-3'

60

Decorin

5'-ATGACCCTGACAATCCCCTG-3'

5'-CCCAGATCAGAACACTGCACC-3'

60

Scleraxis

5'-GGACCGCAAGCTCTCCAAG-3'

5'-ACCCACCAGCAGCACATTG-3'

62

Sox9

5'-GCAGACCAGTACCCGCATCT-3'

5'-CTCGCTCTCGTTCAGCAGC-3'

62

Adipocyte-specific

ADD1

5'-CAGTGACTCTGAGCCCGACA-3'

5'-ATGCCTCGGCTATGTGAAGG-3'

61

PPARγ

5'-ATCATCTACACGATGCTGGCC-3'

5'-CTCCCTGGTCATGAATCCTTG-3'

59

SCD1

5'-ACACCATGGCGTTCCAAAAT-3'

5'-CGGCGTGTGTTTCTGAGAACT-3'

61

C/EBPα

5'-CGCAAGAGCCGAGATAAAGC-3'

5'-GCGGTCATTGTCACTGGTCA-3'

60

GLUT-4

5'-ATGGCTGTCGCTGGTTTCTC-3'

5'-ACCCATAGCATCCGCAACAT-3'

59

AP2

5'-TGATGCCTTTGTGGGAACCT-3'

5'-GCAAAGCCCACTCCCACTT-3'

58

acrp30

5'-AAGAAGGACAAGGCCGTTCTC-3'

5'-GAGGAGCACAGAGCCAGAGG-3'

60

LPL

5'-CCAATGGAGGCACTTTCCAG-3'

5'-CCACGTCTCCGAGTCCTCTC-3'

60