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Table 1 Wnt-1 induced gene database. This table summarizes the list of putative Wnt-1 responsive clones identified in the screen, including accession numbers of genes to which the sequences have homology, as well as the results obtained for each clone by Northern blot and dot blot analyses (in some cases for the latter, results from two experiments are indicated in the "Results – dot blot" column and are separated by a comma). Signals were quantified using a PhosphorImager. The value in the "Results" column for "Northern Blot" is the measure of the ratio of the signal for each gene in the Wnt-1 cells (normalized to GAPDH) to the signal for each gene in the control cells (normalized to GAPDH). The table also indicates whether the gene has been confirmed by semi-quantitative RT-PCR analysis as a true Wnt-1 responsive gene. A description of the function of each gene is listed in the last column of the table. "nd" means that no signal was detected. "inc" means increase in gene expression in the Wnt-1-treated sample, with no detectable signal in the control.

From: Identification of Wnt responsive genes using a murine mammary epithelial cell line model system

   

Results

 

Clone Having Homology to:

Accession Number

RT/PCR

Dot Blot

Northern Blot

Descriptors

Activin

AF41202

+

3.7, inc.

3.2

Mesderm determination factor

Cul-2

U83410

+

both inc.

2.6

Cell cycle exit (cullin)

DUB1

NM 007887

+

both inc.

2.2

Ubiquitin C terminal hydrolase

FAF1

U39643

+

inc., 1.9

2.4

Fas-associated factor (apoptosis)

FIN16

U42385

ND

4.5, inc.

2

NTP binding protein related, FGF4 inducible

FKBP12

BC043920

+

59.9, inc.

nd

FK506 binding protein (cytoplasmic)

GKLF

AF117109

ND

3.8, inc.

4.2

Kruppel-like trxn factor (gut)

Histone H3.3

X13605

+

23.5, inc.

7

In quiescent, differentiated cells

Thymosin β4

U38967

+

5.9, inc.

8

Sequesters actin monomers

Tob5

D38305

+

6.8, inc.

8

p185erbB2, no proliferation

Cyclin G

AB005559

+

both inc.

2.7

p53 target gene, inc in G1/S

EI24

U41751

+

both inc.

1.3

Ectoposide-induced RNA 2.4 kb

IGF BP5

L12447

+

7.3, inc.

1.2

Myoblast differentiation-binds IGF

RNA Helicase

L25126

ND

4, inc.

5.2

8 proteins, unwind dsRNA

SCID Comp. C8

D78188

ND

0.94

nd

Complementing gene 2

Thrombospondin

M87276

+

1.4

nd

Angioenesis inhibitor

URE B1

BC054373

+

both inc.

nd

Y phosphorylated nuclear protein; inhibits p53

Calcineurin

M81475

+

inc., 4.0

4.6

protein phosphatase (S/T)

Caldesmon

U18419

+

8

1.8

Ca/CaM, actin binding protein of muscle

Calmodulin

M19381

ND

both inc.

3.2

Ca binding protein, signaling

Calponin

BC061809

ND

both inc.

2.7

Binds F-actin, CaM, tropomycin

Ribosomal Protein L19

BC020315

ND

0.68

3.3

Breast cancer overexpression (erbB-2)

BTEB2

AB030824

+

both inc.

8.8

GC-box binding protein, trxn factor

E2F1

L21973

ND

1.4

nd

Trxn factor for S phase, Rb binding protein

Elf1

AF016040

+

3.8, inc.

1.5

Ets-like factor, trxn factor

HMG14

M94283

ND

2.4

nd

Chromatin factor for trxn

HP1.BP38

X19643

ND

50.8, inc.

nd

TIF1 binding protein, trxn

NonO

BC005465

ND

1.8

nd

NonPOU, octamer binding protein

Tlm

X53493

ND

1.4

nd

Mouse oncogene, RT homolog

Annexin VII

L13129

ND

both inc.

nd

GTP binding protein, vesicle fusion

ARF4

D87901

ND

2.6

4.4

GTPase, recruits coat proteins

Human GAP

M23612

ND

0.76

nd

GTPase activating protein

Rat GAP

L13151

ND

1.8

nd

GTPase activating protein

GDP Dissoc. Inhib. 2

NM 021494

ND

inc., 11.5

1.6

Rab6 binding protein

GDP Dissoc. Inhib. 2

NM 021494

ND

inc., 11.5

1.6

Rab6 binding protein

Rab11b

BC066656

ND

4.6

1.7

YPT/Rab, vesicle transport

Rab14

NM 053589

ND

16.4, inc.

nd

LMW GTP binding protein

Rap1B

U07795

ND

3.2, inc.

nd

Small GTP binding protein, like Ras

RhoB

M74295

ND

both inc.

1.3

Small GTP binding protein, actin filament functions

Actin-like protein

D12816

ND

0.35

1.9

ECM/Cytoskeletal genes

Gamma/Beta actin

M12481

ND

0.85

7.4

Cytoskeleton architecture

Basigin

BC010270

ND

0.85

1.8

Ig glycoprotein, associated w/integrins

Collagen alpha1 (VIII)

AB009993

ND

2.7, inc.

2.6

Minor ECM, inc in breast carcinomas

Collagen (XII)

U25652

ND

both inc.

8.8

Collagen associated protein

Connexin 43

M61896

+

both inc.

24.6

Gap junction protein

Destrin

BC009477

ND

2.2

2.8

Cofilin isoproein, regulates actin

Fibronectin Receptor

BC059829

ND

3.6

nd

a6b1 integrin

N-cadherin

M31131

+

1.8

nd

Cell adhesion, neural

Radixin

X60672

ND

6.2, inc.

11.7

Links actin to plasma membrane, ERM

SPARC

BC004638

+

1.7

2.4

ECM breakdown, secreted glycoprotein

Syndecan-2

BC047144

ND

inc., 1.7

3

Heparan sulfate glycoprotein, near myc on chromosome

Vinculin

BC039174

ND

2.2

2

FA/ZA junctions, related to a-catenin

ERK3

M64301

+

3.7, inc.

1.3

S/T kinase, Y phosphorylated (by insulin/NGF)

BiP

BC020235

ND

0.8

1.4

Binds Wnt-1 in ER

Nucleoporin

U63839

+

both inc.

nd

p58, nuclear protein transport

Prot. Phos'tase V

X77237

ND

2

7.6

S/T, tetratricopeptide repeats

Prot. Phos'tase Inhib.-2

L20858

+

82.8, inc.

nd

Regulated type I phosphatase

Prot. Phos'tase I (Dis2m2)

M27073

ND

inc., 2.7

2.7

Yeast chromosomal disjoining

PTPase 2

S52655

ND

both inc.

nd

Protein Y Phosphatase, T cells

Tra1

D78355

ND

inc., 1.8

1.8

Ca binding protein, cell surface glycoprotein

Thymopoietin

U39078

ND

1.3, inc.

2.4

Nuclear protein, LAP2 sequence similarity

Proteasome.Comp.C8

D90258

ND

5.5

nd

Subunit of human proteasome

Proteasome.IOTA

D10755

ND

0.85

2.4

Subunit of human proteasome

Wrch-1

AF378088

+

3.2

4.8

Cdc42 homolog

Wnt-5A-like gene

M89798

+

4.8

8.4

Wnt-5A homolog

cdk4-like binding protein

L01640

+

2

2.5

cdk4 binding protein

Fat-like gene

AJ250768

+

5.8

1.7

Fat gene

WISP-1

AF100777

+

58.6

3.7

cyr61/CCN family

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BMC Developmental Biology

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