Figure 9

Increased epidermal expression of E-cadherin correlates with abnormal positioning of embryonic PNS neurons. Stage 16 wild type embryos (A-C) or maternal and zygotic spen mutant embryos (D-F) were generated as described in Materials and Methods. The expression of the PNS neuronal marker 22C10 (green) and E-cadherin (red), were detected with specific MAbs followed by fluorescein conjugated anti-mouse antiserum and a Cy3 conjugated anti rat as described earlier in Figure 4, and in the Material and Methods section.