Figure 8
Abnormal positioning of PNS neurons in spen maternal and zygotic mutant embryos. Maternal and zygotic spen mutant embryos were obtained as described in Materials and Methods, and at stage 14–15 were stained for the neuron specific marker Elav, together with an anti β-galactosidase antiserum to reveal the presence of the CyO, wg-lacZ balancer in heterozygotes (not shown), followed by biotinylated secondary antibodies, and streptavidin conjugated horse radish peroxidase. Brown staining reveals the nuclei of PNS neurons in wt (A, B), maternal and zygotic spen231 (C, D), or spen361 (E, F) embryos.