|
Gene
|
Induces or represses dauer?a
|
Fold regulationb
|
p value
|
|---|
|
insulin pathway
|
|
daf-2 (receptor)
|
strongly represses
|
-3.1
|
<0.0001
|
|
akt-2
|
represses
|
-3.0
|
<0.0001
|
|
akt-1 allc
|
represses
|
1.0
|
ns
|
|
akt-1ac
|
unknown
|
-1.8
|
<0.003
|
|
akt-1bc
|
unknown
|
1.4
|
<0.03
|
|
pdk-1
|
represses
|
1.8
|
<0.0001
|
|
daf-16ad
|
unknown
|
1.0
|
ns
|
|
daf-16 alld
|
promotes
|
-1.7
|
<0.0004
|
|
Insulin-like ligands
|
|
ins-4
|
repressese
|
-2.1
|
<0.002
|
|
ins-5
|
unknown
|
-2.6
|
<0.02
|
|
ins-6
|
repressese
|
-2.6
|
<0.02
|
|
ins-7
|
represses
|
-2.1
|
<0.005
|
|
ins-18
|
promotese
|
1.8
|
<0.002
|
|
ins-33
|
unknown
|
3.9
|
<0.0003
|
|
ins-35
|
unknown
|
6.5
|
<0.0001
|
|
insulin receptor-like
|
|
F56A4.C
|
unknown
|
-3.5
|
<0.0001
|
|
Y19D10A.7
|
unknown
|
-2.8
|
<0.0003
|
|
F14D2.6
|
unknown
|
1.8
|
<0.006
|
|
F15E11.11
|
unknown
|
1.8
|
<0.008
|
|
other dauer and aging regulators
|
|
daf-12
|
strongly induces
|
2.2
|
<0.0007
|
|
daf-5
|
induces
|
-2.0
|
<0.0003
|
|
daf-9
|
represses
|
-4.2
|
<0.02
|
|
scl-1
|
unknown
|
5.2
|
<0.006
|
- ns, not significant This
- table shows all insulin-like ligands, insulin-receptor-like genes, TGFβ, and insulin/IGF pathway genes with greater than 1.8-fold regulation and a p value less than 0.05. Genes checked for regulation that showed <1.8 fold regulation or p > 0.05 or both: clk-1, clk-2, sir-2.1, daf-11, daf-7, daf-1, daf-4, daf-14, daf-8, daf-3, daf-19, tax-4, npc-1, daf-18, age-1, old-1, mev-1, hsf-1, daf-21, gpa-2, gpa-3, kin-29, kin-8, unc-3 and tph-1, as well as all genes annotated as insulins present on the microarray (ins-2, ins-3, ins-11, ins-17, ins-22, ins-23, ins-24, ins-26, ins-30, ins-32, ins-34, ins-37) and nearly all genes identified as putative insulin receptors in Dlakic [27].
- aInduction or repression of dauer is defined by mutant or RNAi loss-of-function phenotypes unless otherwise noted. daf-9 mutants have characteristics similar to both types, and are thus marked "mixed".
- cThe fold change between mutant dauers and wild-type. A positive number indicates higher expression in dauers, a negative number indicates higher expression in N2.
- cThe table entry "akt-1 all" is for a probe that recognizes both isoforms. The other two akt-1 entries each cover an exon unique to the indicated isoform.
- dThe entry "daf-16a isoform" is for a probe that recognizes:R13H8.1c (daf-16a1), R13H8.1b (daf-16a2), and R13H8.1d, but not R13H8.1a (daf-16b) or R13H8.1e. The entry "daf-16 all" is for a probe that recognizes all five isoforms of daf-16.
- eOverexpression of ins-4 or ins-6 represses dauer in a daf-28 mutant [7]. ins-6 has also been shown biochemically to act as a receptor agonist [25]. Overexpression of ins-18 promotes dauer [24].
