Figure 4

Regulation of dFOXO through insulin signaling is conservedbetween mammals and flies. The GMR-Gal4 driver wasused to drive the expression of (B) dPI3K-DN, (C) wild type dPI3K,(D) dAkt, (E) dFOXO, (I) mFoxo1, and (M) mFoxo1-AA, both alone andin various combinations (F-H, J-L, N-P) as indicated through therows and columns in the figure (scale bar = 150 μm). Genotypes are:(A) w; GMR-Gal4/+, (B) w; UAS-dPI3K-DN/GMR-Gal4,(C) w; UAS-dPI3K/GMR-Gal4, (D) w; UAS-dAkt/GMR-Gal4,(E) w; GMR-Gal4/+; UAS-dFOXO/+, (F) w; UAS-dPI3K-DN/GMR-Gal4;UAS-dFOXO/+, (G) w; UAS-dPI3K/GMR-Gal4; UAS-dFOXO/+,(H) w; UAS-dAkt/GMR-Gal4; UAS-dFOXO/+ (I) w; GMR-Gal4,UAS-mFoxo1/+, (J) w; GMR-Gal4, UAS-mFoxo1/UAS-dPI3K-DN,(K) w; GMR-Gal4, UAS-mFoxo1/UAS-dPI3K, (L) w; GMR-Gal4,UAS-mFoxo1/UAS-dAkt, (M) w, UAS-mFoxo1-AA/w; GMR-Gal4/+,(N) w, UAS-mFoxo1-AA/w; GMR-Gal4/UAS-dPI3K-DN, (O) w,UAS-mFoxo1-AA/w; GMR-Gal4/UAS-dPI3K, (P) w, UAS-mFoxo1-AA/w;GMR-Gal4/UAS-dAkt.