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Figure 2 | BMC Developmental Biology

Figure 2

From: IfkA, a presumptive eIF2α kinase of Dictyostelium, is required for proper timing of aggregation and regulation of mound size

Figure 2

Detection of ifkA and ifkB mRNA levels. Total RNA was isolated from growing cells (0) and at the times indicated (in hours) after the onset of development. RT-PCR was carried out using oligonucleotides that detect both ifk mRNAs using RNA from the parental strain, Ax4 (panel A) or RNA from the ifkA null strain, BS153 (panel B). Oligonucleotides specific for ifkA also were used with RNA from Ax4 (panel C), and these primers gave no band when used with RNA from BS153. The picture for panel C was taken at a longer exposure time than the other two panels in order to reveal the faint bands present during development. H7 specific oligonucleotides were used as controls (H7 is a constitutively expressed gene), and the H7 band was similar in intensity for all lanes (panel D, Ax4 RNA; panel E, BS153 RNA).

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BMC Developmental Biology

ISSN: 1471-213X

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