Stromelysin-1 and mesothelin are differentially regulated by Wnt-5a and Wnt-1 in C57mg mouse mammary epithelial cells

Table 1 Genes differentially regulated by Wnt-5a/Rfz-2 and Wnt-1 in C57mg cells.

nd, not determined
		Avg. fold changes
Functional Category of Genes	GenBank acc. no.	Wnt-5a Rfz-2		Wnt-1
		Microarray	Quant. RT-PCR	Microarray	Quant. RT-PCR
Cell Growth/Differentiation
BMP-4	L47480	-3	-2.4	1.4	1.2
Cell Adhesion
plasminogen activator inhibitor-1	M33960	-2.8	-2.4	2.1	1.2
osteoblast specific factor 2	D13664	-2.4	-1.6	1.4	1.1
Flt4 ligand	U58112	3.3	2	1	1.2
fibronection	ET62894	2	nd	-1.8	nd
HTK ligand	L38847	-2.1	-1.9	2.5	3.2
mesothelin	D86370	-4	-2.9	8.4	2.8
stromelysin 1	X66402	3.8	4.4	-1.6	-2.5
Transcriptional Regulator
groucho-related gene 1 protein	U61362	-2.1	-1.6	1.4	-1.4
retinoic acid receptor-alpha	X57528	-2.3	-2	1	-1.3
Id4 helix-loop-helix protein	X75018	-4.1	-3.6	1.6	-1.1
neural-restrictive silencer factor	U13878	-3.5	1.1	1.6	1.8
bcl-3	M90397	1.9	2.2	-2	-1.4
Krox-20	X06746	-3.4	-1.7	6.2	1.5
Inflammation
PTX3	X83601	-2.5	-2.1	1.6	1.6
bradykinin B1 subtype receptor	U47281	-2.5	4	2.7	3.2
Signaling
Rho-associated protein kinase	U58513	-1.2	nd	-4.9	nd
guanine nucleotide-binding protein	W83658	1.6	1.7	-4.4	-4.4
signaling molecule (ATTP)	U97571	-1.3	1.5	-3.5	-2.7
son of sevenless 1	Z11574	2.5	2.9	-2.6	-2
SHPS-1	D87967	-2.7	-1.4	3	-1.7
TRAF1	L35302	2.6	1.5	2	1.7
Cell surface receptor
interleukin 2 receptor gamma	U21795	2	2.7	-1.4	1.9
transferrin receptor	X57349	-1.6	-1.6	-2.3	-1.9
Cytokine
interleukin-6	X54542	2.1	-1.1	1.6	1
Cell Cycle
Ki-67 antigen	ET62993	-1.45	-1.1	-6.5	-5.8
Axon Guidance
semaphorin F	X97817	2.5	3.1	-2	-4
DM-GRASP	L25274	3.3	1.8	-2.8	-2.6

Acc., accession; Avg., average; Quant., quantitative; BMP-4, bone morphogenetic protein-4; HTK ligand, hepatoma transmembrane kinase ligand; PTX3, pentraxin 3; SHPS-1, src homology protein substrate-1; TRAF1, TNF receptor associated factor-1. Shown is a list of genes that are differentially regulated by Wnt-5a/Rfz2 and Wnt-1 in C57mg cells identified by DNA microarray analysis. The values determined by DNA microarray analysis were validated using quantitative RT-PCR. Average fold changes from DNA microarray analysis and quantitative RT-PCR were determined from two independent experiments and from three independent experiments performed in triplicate respectively.

ISSN: 1471-213X