Figure 4
SPE-9 is concentrated in spikes during spermiogenesis and pseudopods in mature sperm. (A, C, E, G) Nomarski DIC images. (B, D, F, H) Staining for SPE-9 in red. Arrows point to spikes or pseudopods. (A, B) Wild-type spermatid activated with TFP. (C, D) spe-12(hc76) spermatid treated with pronase. Note the numerous small spots that correspond to arrested or developing spikes. (E, F) Wild-type sperm activated by TEA treatment and stained with anti-SPE-9-EX. (G, H) Wild-type sperm activated by TEA treatment and stained with anti-SPE-9-C. Bar in G is 5 μm and applies to all panels.