Figure 4

Removing the lower jaw does not restore the reorientation of the palatal shelves in Wnt1-Cre;Ldb1^fl/-mutants. The upper jaw region from E13.5 embryos, before culture (A-D) and after 3-day culture in the absence of the lower jaw and the tongue (E-N). A-H are fluorescent pictures of the heads processed by whole mount DAPI staining. (A,C,E,G) Intra-oral views directly into the palate. (B,D,F,H) Oblique views showing the profile of the palatal shelf. Red arrows in F and H point to the posterior palatae. (I-N) Coronal sections were stained with Nuclear Fast Red. Abbreviations: ant, anterior; pos, posterior; No, nose. Bar, 0.5 mm.