Targeted germ line disruptions reveal general and species-specific roles for paralog group 1 hox genes in zebrafish

Table 2 Characteristics of hoxb1a and hoxb1b germ line mutant alleles

Founder	Transmission frequency	Sequence^a	Type of mutation^b	ID^c
*hoxb1a*		TTTGTAACCGTGGGACGAACGCCTACT
A2	14%	TTTGTAAC GTGGGACGAACGCCTACT	Deletion (−1 bp)	um189
		TTTGTACACAATTTGTACAATTTGGACGAACGCCTACT	Insertion/deletion (+17 bp)	um190
		TTTGTACGGG GGACGAACGCCTACT	Insertion/deletion (−5 bp)	um191
		TTTGTACTCCATTTGTA CTACT	Insertion/deletion (−5 bp)	um192
A20	9%	TT TGGGACGAACGCCTACT	Deletion (−8 bp)	um193
		TTTGTAATTTC GGACGAACGCCTACT	Insertion/deletion (−2 bp)	um194
*hoxb1b*		ATCGCAGCCCTTCCACCTCAACGTGGACATGGG
B2	45%	ATCGCAGCCCTTCCACCTCAACGTGGACATGGG	Deletion (−8 bp)	um195
B11	43%	ATCGCAGCCCTTCCACATTCC GTGGACATGGG	Insertion/deletion (−1 bp)	um196
B15	41%	TGTTTCAATCGTGAAACACAAATTCACAAATTCAATCGTGGACATGG	Insertion/deletion (+19 bp)	um197

^aSequence of mutant alleles. Deletions are shown as gaps and insertions are italicized. Nucleotides in bold indicate target site for diagnostic restriction enzyme in wild type sequence.
^bIndicates whether mutation results from insertion, deletion or both. Numbers in parenthesis indicate net gain/loss of nucleotides in mutant sequence.
^cIdentifying designation for each mutant allele.

ISSN: 1471-213X