Targeted germ line disruptions reveal general and species-specific roles for paralog group 1 hox genes in zebrafish

Table 1 Characteristics of TALE and zinc finger nucleases targeting hoxb1a and hoxb1b

TALEN/ZFN	Target coordinate^a	Upstream TALEN/ZFN	Downstream TALEN/ZFN	Gap^c	No. of units^d	Embryos injected^e	Activity^f	Diagnostic enzyme^g
TALEN/ZFN	Target coordinate^a	Target sequence^b	Target sequence^b	Gap^c	No. of units^d	Embryos injected^e	Activity^f	Diagnostic enzyme^g
Zb1b-1	Chr12:28712770	GGTGGAAGG	GTGGACATG	5bp	3	274	NO	BslI
Zb1b-2	Chr12:28712770	GGTGGAAGGGCT	GTGGACATGGGT	5bp	4	424	NO	BslI
Zb1b-3	Chr12:28712770	GTGGAAGGGCTG	GTGGACATGGGT	6bp	4	109	YES	BslI
Zb1b-4	Chr12:28712773	GAGGTGGAAGGG	GACATGGGTAAA	6bp	4	149	YES	BslI
Zb1a-1	Chr3:24060660	GCTGATAAG	GATGCGAAG	6bp	3	2916	NO	FatI
Zb1a-2	Chr3:24060660	GCTGATAAGATG	GATGCGAAGGCC	6bp	4	541	NO	FatI
Zb1a-3	Chr3:24060602	GCCATAGTGTGG	GCCGGTGCGTAC	6bp	4	72	NO	BslI
Zb1a-4	Chr3:24060535	AGGGTTGATAAA	GGATGGGATGTA	5 p	4	56	NO	BslI
Tb1a-1	Chr3:24060209	TCCAGAATGAACTC	TCCCACGGTTACAAAT	16bp	15/16	50	NO	RsaI
Tb1a-2	Chr3:24060227	CTTGGAGTACACAAT	TGGGCGAGTAGGCGTT	16bp	16/16	50	YES	BtgI
Tb1a-3	Chr3:24060213	CCAGAATGAACTCTTTC	TCGTCCCACGGTTAC	16bp	18/15	50	NO	RsaI

^aTarget coordinate as defined by midpoint between the upstream and downstream nuclease recognition sites.
^bGenomic sequence targeted by the upstream and downstream TALE and ZF nucleases.
^cDistance between upstream and downstream TALE and ZF nucleases.
^dThe number of TAL or ZF units used to assemble each nuclease.
^eThe number of embryos injected to test activity and raise mutant families.
^fNucleases that disrupted the diagnostic restriction site were defined as active.
^gThe enzyme used to test activity of nucleases.

ISSN: 1471-213X