Figure 2

CLIC4 expression in embryos. 13.5. 15.5 and 18.5 days post coitus (pc) homozygous CLIC4-GFP embryos were used for the study (A-D). GFP staining of 13.5 pc WT embryo is shown as a control. All embryos were stained with an antibody against GFP. CLIC4-GFP is illustrated in B-D for Pallium (Pall), alar plate of thalamus (AT), commissural pretectal region (CoP), paraseptal subpallium (PaSe), collicular midbrain tectum (CMT), basal part temporal hypothalamus (HyB), prepontine hindbrain (PPH), medullary hindbrain (MH), dorsal pallium (DPall), ventral pallium (VPall), DPall ventricular (DPallv), medial pallium (MPall), DPall intermediate stratum (DPalli), olfactory bulb (OB), regions of the central nervous system, as well as for tongue (T), tooth primordium (TP), larynx (La), cardiac ventricle (V) and atrium (At), aorta (Ao), lung (L), liver (Li), kidney (K), intestines (I), stomach (S), spinal cord (SC), cortical mesonephros (M), turbinates (Tu), Meckel’s cartilage (MC), pancreas (P), genital tubercle (GT), salivary gland (SG), thymus (Th), urachus bladder (UB) and brown fat (BF). GFP staining of integument changes at the time of stratification of the epidermis in 15.5 and 16.5 days old embryos (E and F) (str-stroma/dermis, bc-basal cell, sbc- suprabasal cell, hfb- hair follicle bud). CLIC4 expression as detected by anti-GFP immunohistochemistry in embryonic tissues from a E13.5 day and E18.5 day developing mouse (G and H). Note that while developing kidney (K) maintains similar expression over this period of development, CLIC4 expression diminishes in myocytes (M), spinal cord (SC), and chrondrocytes vertebrae (V). Hematoxylin counterstain. Bar = 50 μm.