Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6

Table 3 Decreases in pJW3.9::GFP expression in elt-6 and egl-18 mutants in embryonic P cells

Background	RNAi	N	% eggs with WT expression in P5-P8
Wild-type	-	50	98%
Wild-type	Control	36	94%
Wild-type	*elt-6*	50	78%*
Wild-type	*egl-18*	36	91%
*elt-6(gk723)*	Control	34	56%*
*elt-6(gk723)*	*egl-18*	36	58%*
*egl-18(n162)*	Control	38	68%*
*egl-18(n162)*	*elt-6*	36	69%*
*egl-18(ga97)*	Control	20	60%*
*egl-18(ga97)*	*elt-6*	24	54%*

Animals containing pJW3.9::GFP in either wild type, elt-6 mutant (gk723) or egl-18 mutant (n162 and ga97) backgrounds were synchronized and given the indicated RNAi treatment. pJW3.9::GFP expression in P5 - P8 was scored at bean stage in the embryos laid by these animals. ‘N’ = number of embryos scored. The percentage of animals having a wild-type intensity of GFP in all four P cells based on visual observation is indicated in column four. ‘*’ indicates P < 0.05 compared to control (Fisher’s exact test).

ISSN: 1471-213X