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Figure 1 | BMC Developmental Biology

Figure 1

From: Multiple transcription factors directly regulate Hox gene lin-39 expression in ventral hypodermal cells of the C. elegans embryo and larva, including the hypodermal fate regulators LIN-26 and ELT-6

Figure 1

LIN-39 regulators identified in yeast bind to lin-39 promoter regions in vitro . A - F) Gel mobility shift assays with proteins expressed and purified from E. coli. Arrowhead indicates free probe; arrow indicates protein DNA complexes. For each panel, the top line identifies the labeled probe used, the bottom line shows the amount of protein added in each lane; middle lines (panels A, B, D and F) indicate the identity and amount of competitor added. A) NHR-43 binds to YF1 (342 bp). Lanes 3–5 contain cold wild type YF1 as competitor, while lanes 6–8 contain cold YF1 with the TGAC site mutated as competitor; B) ALR-1 binds to ECR2 (40 bp); this binding is competed by wild type ECR2 but not by a scrambled oligonucleotide with the same nucleotide composition (ECR2S); C) ZTF-17 binds to YF4-2 (186 bp) and YF4-4 (110 bp); D) ZTF-17 binding to YF4-2 is competed by YF4-4 but not YF4-3; E) LIN-26 binds to YF4-3-1 (51 bp); F) LIN-26 binding to YF4-3-1 is competed by YF4-3-1 (51 bp) but not YF4-3-2 (52 b); G) Fragment YF4 with ECRs 7–10 is shown above, with smaller subfragments diagrammed below. Shading indicates fragments that were bound by ZTF-17 and/or LIN-26 in yeast one-hybrid assays and in vitro.

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BMC Developmental Biology

ISSN: 1471-213X

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