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Figure 5 | BMC Developmental Biology

Figure 5

From: Primary culture of avian embryonic heart forming region cells to study the regulation of vertebrate early heart morphogenesis by vitamin A

Figure 5

Retinoic acid regulates the expression of FN1 and NEO in chicken heart forming cell culture. Primary heart forming region cells from 3–5 ss chick embryos were cultured for 3 d in either DMEM or Endo-Grow (Endo) media in the presence or absence of retinoic acid (RA). (A) Relative quantification of FN1 mRNA in comparison to FN1 mRNA in control cells with no added RA. (B) Relative quantification of NEO mRNA in comparison to NEO mRNA in control cells with no added RA. Standard deviations are indicated by error bars. The differences in comparison to the control at significance level p < 0.05 are marked by (*); at p < 0.005, by (**); at p < 0.0001, by (***).

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BMC Developmental Biology

ISSN: 1471-213X

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