Figure 5
From: Histone modifications in the male germ line of Drosophilaa
Overviews of label in the testis tube of D. melanogaster.(a-c) H4K12ac; different stages of primary spermatocytes show the gradual decondensation of the chromatin. In older spermatocytes (Stc(o)) mainly X chromosome and autosomes as well as the nucleolus are labeled. Younger spermatocytes (Stc(y)) and spermatogonia (Stg) have a more compact chromatin. The signal strength increases from spermatogonia to young spermatocyte stages (compare DAPI with antibody fluorescence!). (d-f) H3K9me3; apical tip of the testis tube. Spermatogonia are only slightly labeled and the cytoplasm seems slightly labeled (compare (e) with spermatogonia in (b)!). The hub cells (hub) display a strong reaction. Spermatocytes (Stc) show a more diffuse label compared with (b), as is characteristic for Y chromosomal chromatin label (cf. Figures 4d-i). Cytoplasm is unlabeled. (a, d) DAPI; (b, e) antibody; (c, f) merged pictures of DAPI and antibody.