Figure 7
Hypothyroidism leads to decreased phosphorylated Cofilin. (A) Representative western blots for protein isolated from embryonic day 16 (E16) cochleae show decreased intensity of p-Cofilin in hypothyroid conditions relative to control. Total Cofilin appears to be unchanged. Average relative density (mean ± s.e.m.) of phosphorylated, labeled proteins indicates decreased expression of p-Cofilin in hypothyroid cochleae. Average relative density of Cofilin is not significantly different between control and hypothyroid. (B) At postnatal day 0 (P0), intensity of p-Cofilin in hypothyroid conditions appears to be decreased relative to control conditions. Average relative density of p-Cofilin is significantly decreased in hypothyroid relative to control conditions (*p-value < 0.05). (C) By postnatal day 3 (P3), the relative intensity and average relative density of p-Cofilin labeled protein is unchanged between control and treated conditions. As for E16, total protein level of Cofilin is not significantly different between control and hypothyroid cochleae at P0 and P3.