Figure 10
From: c-kit expression profile and regulatory factors during spermatogonial stem cell differentiation
Expression of c-kit and other germ cell differentiation-related genes in the c18-4 and CRL-2053 cells stimulated by RA. The c18-4 and CRL-2053 cells were treated with 2 μM RA diluted in ethanol for 24 h in vitro. Cells treated with the same concentration of ethanol without RA were the control group. Realtime PCR was carried out for quantitative determination of the expression of c-kit and its potential regulatory genes (Bmp4, Cyp26b1, Dazl, Egr3, Pou5f1 and Stra8). Values of the vertical axis represented the expression fold change comparing with the control group. The results were normalized to GAPDH values. Error bars represent the S.E.M. Values with ** represented a significance with a P<0.01 whereas values with * represented a significance with a P<0.05 comparing with the control group. (A) c-kit expression in the c-18-4 cells. (B) c-kit expression in the CRL-2053 cells. (C) Expression of germ cell differentiation-related genes in the c18-4 cells. (D) Expression of germ cell differentiation-related genes in the CRL-2053 cells.