Figure 7
OCT4 and NANOG distribution pattern in bovine hatched blastocysts (9dpi). The top panel (a) of images shows a 3-D reconstruction of confocal sections taken through bovine HBL. Dashed line encircles the ICM. The bottom panel (b) represents a single optical section through the ICM of the same embryo. OCT4 and NANOG localise in the ICM, but a certain degree of signal segregation was observed. Cells indicated with red arrowheads express NANOG and cells indicated with white arrowheads co-express both proteins. DAPI marks chromatin. Confocal sections were taken every 3 μm.