Figure 7

Crol is enriched across consensus zinc finger binding sites in the wg promoter. (A) - 6 overlapping primer sets spanning 5 kb of the wg promoter, wg transcription start site +1. (B) - region of wg promoter containing overlapping amplicons for wg (Wg primers 1–6) used for ChIP with anti-Crol or Ci, the boxed region highlights amplicons positive for Crol enrichment (see gel in C) and the dashed grey line shows the region of the wg promoter containing the zinc finger and Ci consensus sites shown in 7B. (B) - Position of primers for ChIP-qPCR (Wg2.1-Wg2.4) are marked with arrows. The in vitro defined Ci binding site and consensus zinc finger binding sites are boxed and labelled. (C) – DNA gel electrophoresis of ChIP-PCR across the Wg promoter using Wg primers (as marked in 7) for Input DNA; Ci ChIP or Crol ChIP as marked. (D-E) Fine mapping of Crol and Ci enrichment using ChIP-qPCR for small amplicons - Wg2.1-2.4 shown in (B). (D) - ChIP for Crol - percentage enrichment and error for percentage enrichment for Crol are as follows; Wg 2.1 (0.28 ± 0.0045); Wg 2.2 (0.453 ± 0.0083); Wg 2.3 (0.055 ± 0.0082) and Wg 2.4 (0.00116 ± 0.0095). (E) - ChIP for Ci - percentage enrichment and Error for percentage enrichment for Ci are as follows; Wg 2.1 (0.482 ± 0.0066); Wg 2.2 (1.031 ± 0.0141); Wg 2.3 (0.24 ± 0.0103) and Wg 2.4 (0.137 ± 0.019). (F) - Model for regulation of cell cycle patterning across the anterior wing margin by EcR.