Figure 4

EcR is essential for CycB expression at the wing margin. (A-D) - Pattern of overlap between the Cyclin B-GFP protein trap (CycB-PT) in green (A) and anti-EcR antibody in red (B). The merge is shown in (C) and overlap with DNA in (D). (E-H) - EcR RNAi clones marked with β-gal (red) with CycB-PT (green). (E) - disruption to the Cyc B pattern across the margin marked with white arrows corresponds to the EcR RNAi clones shown with β-gal in F. The merge is shown in G and the presence of cells across the margin lacking CycB shown with DNA in H. (I-L) - Disruption to both CycB-PT activity in green (I) and the G2-marker Geminin in purple (J) for large EcR RNAi clones detected with β-gal in red (K). In (I-K) - the position of two sets of non-EcR knockdown cells is show across the margin to highlight non-autonomous affects of EcR knockdown. The presence of cells across the margin shown with DNA in (L).